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Central Government/Public Sector

TRN :33275009 | 25 Nov, 2024

Tender Value : 0

Howrah - West Bengal

Tender for supply of dengue ns1 ag elisa kit rate to be quoted per test basis 1 the elisa kit should be designed for qualitative detection of dengue ns1 antigen of all 4 dengue serotypes in human serum. 2 the kit shouldbe provided with the following materials and reagents: a anti- ns1 antibody coated breakway mi crowells. desiccantshould be provided for storing the unused microwells which are to be resealed immediat ely. b horseradishperoxidase conjugated anti-ns1 monoclonal antibody with preservatives c chromogeni c substrate in buffer dpositive control in the form of recombinant antigen with preservatives and antibiotic s e negative control in the form ofconfirmed negative human serum with preservatives and antibiotics f c alibrators in the form of recombinant antigenwith preservatives and antibiotics g sample diluents h wash buffer 3 the time required for performing the test fordetection of dengue ns1 antigen by elisa should ran ge between 2-4 hours. 4 the elisa kit for detection of denguens1 antigen should have a sensitivity of >9 0% and a specificity of >95% taking rt-pcr as the gold standard. 5 thekit should have a shelf-life of at lea st 18 months when stored at an ambient temperature of 2 degree c - 8 degreec. 6transportation should b e under cold chain.ai2024-25, item code no.s-60096, sl no.644

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